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Journal of The Korean Society of Emergency Medicine 2004;15(6): 475-486. |
| Expressions of the pERK1/2 and the cFos Proteins at an Early Stage of Transient Global Ischemia-reperfusion Injury in the Hippocampus of Rats |
| Chang Seok Ko, Young Hyun Yun, Hun Cheol Ahn, Jeong Woo Choi, Dae Han Wi, Su Jin Yu, Jae Hwang Park |
| Department of Emergency Medicine, Wonkwang University School of Medicine, Iksan, Korea. ysoojin@wmc.wonkwang.ac.kr |
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| ABSTRACT |
PURPOSE:
This study was to evaluate temporal changes in the expressions of the phosphorylated extracellular-regulated kinase1/2 (pERK1/2), the phosphorylated MAPK/ERK kinase1/2 (pMEK1/2) and the cFos proteins in the hippocampus of rats following transient global ischemia.
METHODS:
Transient global ischemia was induced in the forebrains of Sprague-Dawley rats by using a 4-vessel occlusion for 20 min under anesthetic condition.
Hematoxyline-eosin staining showed typical microscopic findings that represented neuronal cell death in hippocampal CA1 regions 5 days after transient global ischemia.
Four-vessel occlusion-reperfusion produced ischemic injury in major forebrain structures, such as the striatum, the cortex and the hippocampus, in the finding of triphenyltetrazolium chloride (TTC) staining.
RESULTS:
A high density of pERK1/2 immunoreactivity existed in the pyramidal-cell layers of the CA2-3 regions and in the granular-cell layers of the dentate gyrus 5 min after ischemia. Following ischemia, expression of the pMEK1/2 protein showed temporal changes similar to that of the pERK1/2 protein. A significant expression of the cFos protein was noted in the pyramidal-cell layers of the CA2-3 regions and in the granular-cell layers of the dentate gyrus 2 hours after global ischemia.
CONCLUSION:
Intracellular signaling cascades of the ERK or the cFos protein take part in early cellular events in the hippocampus of rats in response to ischemic insult. |
| Key words:
Hippocampus, pERK1/2, pMEK1/2, cFos, Transient global ischemia |
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