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J Korean Soc Emerg Med > Volume 14(1); 2003 > Article
Journal of The Korean Society of Emergency Medicine 2003;14(1): 23-28.
Effects of L-Arginine and 7-Nitroindazole in the Gerbil Model of Global Cerebral Ischemia
Young Jin Cheon, Sung Eun Kim, Yong Jae Kim
1Department of Emergency Medicine, College of medicine, Ewha Womans University, Seoul, Korea. kcssis@ewha.ac.kr
2Department of Neurology, College of medicine, Ewha Womans University, Seoul, Korea.
3Department of Pathology, College of medicine, Ewha Womans University, Seoul, Korea.
ABSTRACT
PURPOSE:
This study was performed to determine whether L-Arginine would improve or worsen the neurologic out-come after ischemic brain injury and whether 7- Nitroindazole (7-NI, inhibitors of neuronal nitric oxide (NO) synthase inhibitor) would improve or worsen.
METHODS:
Five (5) groups (N =11 to 14) of anesthetized gerbils were subjected to 10 min of global cerebral ischemia by means of a bilateral carotid artery occlusion. One group (N =11) was the control group. In a second group (N =12), the animals were pretreated with intraperitoneal L-Arginine (300 mg/kg) one hour before ischemic insult. In a third group (N =12), pretreatment with L-Arginine was performed in the same manner and intraperitoneal 7-NI were given at the time of reperfusion and 2 hours after reperfusion. The animals of a fourth group (N =12) were treated with 7-NI in the same manner without any pretreatment with L-Arginine. The animals in the last group (N =14) underwent sham operations.
RESULTS:
Compared with control group, concomittent treatment with L-Arginine and 7-NI showed no significant improvement in the neurological cell survival rate. Also, group pretreated with L-Arginine only showed a similar out-come. The group treated with 7-NI at the time of reperfusion and 2 hours after reperfusion showed a significant improvement in the neurological cell survival rate compared with the control group and the other two experimental groups (p<0.05).
CONCLUSION:
In the ischemia-reperfusion injury of global ischemia in gerbils, NO from endothelial NO synthase has no important role in the neurological outcome.
Key words: Cerebral ischemia, Nitric oxide, 7-Nitroin-dazole, L-Arginine
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