Effect of post-treatment fluvastatin for hemorrhagic shock in rats

Kim, Oh Hyun; Kim, Soo-Ki; Jung, Soon-Hee; Hwang, Sung Oh; Kim, Hyun; Cha, KyoungChul; Cha, Yong Sung; An, Gyo Jin; Lee, Kang Hyun

J Korean Soc Emerg Med > Volume 33(5); 2022 > Article

Original Article

Journal of The Korean Society of Emergency Medicine 2022;33(5): 506-515.

Effect of post-treatment fluvastatin for hemorrhagic shock in rats

¹Department of Emergency Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea
²Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Korea
³Department of Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea

Correspondence

Kang Hyun Lee ,Tel: 033-741-1612, Fax: 033-742-3030, Email: ed119@yonsei.ac.kr,

Received: September 30, 2021; Revised: December 2, 2021 Accepted: February 8, 2022. Published online: October 31, 2022.

ABSTRACT

Objective:
This study aimed to investigate the biochemical, histologic, and immunologic effects of post-treatment administration of fluvastatin in a hemorrhagic shock (HS) rat model.
Method:
Experimental rats were randomly divided into four groups: control group: no drugs and did not undergo HS; control statin group: fluvastatin 1 mg/kg (no HS); HS group: normal saline after HS; HS+statin group: fluvastatin 1 mg/kg+normal saline after HS. Briefly, HS was induced by femoral arterial catheter blood extraction of 30% of the total blood volume. The mean arterial pressure and heart rate were monitored for 2 hours after starting blood withdrawal. Arterial blood gas, complete blood count, and serum cytokine levels were measured at baseline, 2 hours after HS, and 48 hours after resuscitation. The kidneys, lungs, and small intestines were removed for pathological examination 48 hours after HS.
Results:
At the end of the resuscitation period, the HS and HS+statin groups showed reduced bicarbonate, base excess, and platelet counts, all of which differed significantly from values in the control and control+statin groups. Compared to the control group, the HS+statin group exhibited significantly elevated serum interleukin-10 (IL-10) at 2 hours after resuscitation (P<0.05). Except for IL-10, the group-time interaction was not significant for other cytokine profiles.
Conclusion:
This study demonstrates that post-treatment with fluvastatin after HS increases the production of the anti-inflammatory cytokine IL-10 and affects the cytokine profiles in rats.

Key words: Hemorrhagic shock; Traumatic shock; Cytokines; Hydroxymethylglutaryl-CoA reductase inhibitors